Abstract
Potatoes are crucial to food security and feeding the world’s rising population. However, various biotic and abiotic stress factors, such as late/early blight, bacterial wilt, and necrotic ringspot and cold storage warehouse temperature, pH, and moisture, respectively, affect crop productivity and nutrition. This study aims to investigate the impact of cryopreservation on gene expression in potato tubers, evaluating its feasibility for germplasm conservation and potential contributions to food security. Transcriptome analysis was conducted to identify differentially expressed genes, highlighting those involved in essential metabolic and developmental processes. Potato tubers were cryopreserved and later germinated to explore genetic differences following germination. High-throughput sequencing and analysis were performed on total mRNA from cryopreservation and germination stages. De novo transcriptome assembly was performed on processed raw reads, while BUSCO measured transcriptome assembly quality. The average quality data of 6.5 GB per sample (> Q20) and GC percentage of filtered clean reads were 88.92–89.23% and 43–46%, respectively. BUSCO’s assembly was 85% complete, and raw reads aligned to the assembled transcriptome from 78.7 to 83.2%. Cellular components, molecular function, and biological processes dominated Gene Ontology (GO) terms. Expression analysis revealed differentially expressed genes that showed how cryopreservation can alter gene expression to plan germplasm conservation, as it requires gene information. The differential expression analysis for each stage showed 798 differentially expressed genes (DEGs) (115 upregulated and 683 downregulated), 592 (54 upregulated and 538 downregulated), 923 (149 upregulated and 774 downregulated), and 1035 (649 upregulated and 386 downregulated) in the untreated and chemical treatment samples (1st stage), chemically treated and cryopreserved samples (2nd stage), cryopreserved and germinated samples (3rd stage), and untreated and germinated samples (4th stage), respectively. Photosynthesis and abiotic stress response genes were the most differentially expressed, while genes needed for average plant growth and development, such as citric acid cycle, cell elongation, root size determination, seed germination, and flowering, were the least. This study demonstrates that cryopreservation does not adversely impact essential genes for potato tuber growth, suggesting its viability as a safe method for germplasm conservation and potential use in sustainable potato production and food security strategies.
Graphical Abstract